| BACKGROUND | 2B8 epitope sequence (RDPLPFFPP, CGG GAC CCG TTG CCC TTT TTT CCA CCG) is the part of Deinococcus radiodurans Bacteriophytochromes (DrBphP). This monoclonal anti-2B8 antibody is highly specific to any protein containing minimal original epitope sequence (RDPLPFFPP). This antibody also react evenbetter to a modified epitope sequence (RDPLPAFPP). |
| SOURCE | 2B8 epitope is amino acid (3-11) sequence of Deinococcus radiodurans bacteriophytochrome (DrBphP, 75kDa). Anti-2B8 antibody was generated by immunizing purified DrBphP protein into mouse. |
| PRODUCT | Each vial contains 100 μg IgG (monoclonal) in 200 ml of PBS with <0.1% sodium azide, 30% glycerol Specific to the peptide of RDPLPFFPP or RDPLPAFPP. |
| Control protein | purified 2B8 tagged GFP protein (~28 kDa), use 5~10 ml for western blot. APPLICATIONS Anti-2B8 antibody is recommended for detection of RDPLPFFPP or RDPLPAFPP peptide tagged fusion proteins by Western Blot, starting dilution with 1:5,000 (dilution range is 1:5,000~1:20,000), Immunoprecipition [~5 μg per 100-500 μg of total protein (1 ml of cell lysate)] and solid phase ELISA (starting dilution 1:1,000~1:10,000). Chromatine immunoprecipitation(ChIP) |
| STORAGE | Store at -20℃. Stable up to two year from the date of shipment. Non-hazardous. No MSDS required. |